ACU I (300 U)

Descrizione

CTGAAGNNNNNNNNNNNNNNNN/
GACTTCNNNNNNNNNNNNNN/NN

Source:
An E. coli strain that carries the AcuI gene from Acinetobacter calcoaceticus (S.K. Degtyarev).

Reagents Supplied:
NEBuffer 4 (10X)
S-adenosylmethionine (SAM) (800 X)


Enzyme Properties


Activity in NEBuffers:
NEBuffer 1:50%
NEBuffer 2:100%
NEBuffer 3:50%
NEBuffer 4:100%

When using a buffer other than the optimal (supplied) NEBuffer, it may be necessary to add more enzyme to achieve complete digestion.

Methylation Sensitivity:
dam methylation: Not sensitive
dcm methylation: Not sensitive
CpG methylation: Not sensitive
More information about: Methylation Sensitivity

Heat Inactivation:
65°C for 20 minutes

Survival in a Reaction:
(?) Not recommended for digest over 1 hour.


Reaction & Storage Conditions


Reaction Conditions:
1X NEBuffer 4
Supplemented with 40 ?M S-adenosylmethionine (SAM)
Incubate at 37°C.

1X NEBuffer 4:
20 mM Tris-acetate
50 mM potassium acetate
10 mM Magnesium Acetate
1 mM Dithiothreitol
pH 7.9 @ 25°C

Unit Definition:
One unit is defined as the amount of enzyme required to digest 1 µg of ? DNA in 1 hour at 37°C in a total reaction volume of 50 µl.

Concentration:
5,000 units/ml

Unit Assay Substrate:
? DNA

Storage Conditions:
10 mM Tris-HCl
100 mM NaCl
1 mM Dithiothreitol
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Storage Temperature:
-20°C

Diluent Compatibility:
Diluent B