FECAL COLIFORMS BROTH BASE (500 gr)

For the cultivation and enumeration of fecal coliforms in water by the membrane-filtration technique at a high temperature



Applications
Detection / Cultivation / Enumeration
COLIFORMS IN WATER
Packaging
Suspend 37.1 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Cool to 45-50ºC and aseptically add 2 vials, each vial for 500 ml of the medium, of Fecal Coliforms Supplement (Cat. 6023), previously reconstituted in 5 ml of 1% 0.2 N NaOH solution. Boil for one minute until complete dissolution. Homogenize gently and dispense into Petri dishes. DO NOT AUTOCLAVE.

Fecal Coliforms Supplement (Cat. 6023).

Rosolic Acid ..................... 50 mg

Formula
Lactose 12.5
Tryptose 10
Proteose Peptone N°3 5
Sodium Chloride 5
Yeast Extract 3
Bile Salts N°3 1.5
Aniline Blue 0.1