TRYPTICASEIN SOY AGAR (T.S.A.) Nº2 (500 gr)

For the isolation, cultivation and detection of hemolytic activity of fastidious microorganisms

Applications
Hemolytic Activity
OTHER APPLICATIONS
Packaging
Suspend 40 grams of the medium in one liter of distilled water. Allow to stand for 5 minutes and mix well until a uniform suspension is obtained. Heat with gentle agitation and boil for one minute. Sterilize at 121°C for 15 minutes.



Cool to 45 - 50°C, and add 5 - 10% sterile defibrinated blood, homogenize and pour into Petri dishes. Be careful to avoid bubble formation when adding the blood to the cooled medium and rotate the flask or bottle slowly to create a homogeneous solution



Formula
Tryptone H 15
Sodium Chloride 5
Soy Peptone 5
Bacteriological Agar 15