TTC CHAPMAN AGAR (Lactose Agar TTC with Tergitol 7) (ISO 9308-1:2000) (500 gr)

For the detection and enumeration of E.coli and coliforms in drinking water by membrane filtration technique



Applications
Detection / Cultivation / Enumeration
COLIFORMS IN WATER
Packaging
Suspend 56.15 grams of the medium in one liter of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add 5 ml of TTC 1% Supplement (Cat. 6030) previously reconstituted in 5 ml of sterile distilled water. Homogenize gently and dispense into Petri dishes. DO NOT OVERHEAT THE MEDIUM.



TTC 1% SUPPLEMENT (CAT. 6030)

1 Vial reconstructed in 5 ml of distilled water



Triphenyltetrazolium Chloride ……………………………50 mg



Formula
Lactose 20
Peptone 10
Yeast Extract 6
Beef Extract 5
Sodium Heptadecyl Sulfate (Tergitol 7) 0.1
Bromothymol Blue 0.05
Bacteriological Agar 15