YERSINIA SELECTIVE AGAR BASE (500 gr)

Selective medium for Yersinia enterocolitica used with supplement

Applications
Isolation
YERSINIA
Packaging
Suspend 29 grams of the medium in 500 ml of distilled water. Mix well and dissolve by heating with frequent agitation. Boil for one minute until complete dissolution. Sterilize in autoclave at 121ºC for 15 minutes. Cool to 45-50ºC and aseptically add one vial of Yersinia selective supplement (Cat. 6033), previously reconstituted in 5 ml of sterile distilled water. Homogenize gently and dispense into Petri dishes.



Yersinia Selective Supplement (CIN) Cat. 6033

(Composition: each vial for 500 ml)



Cefsulodin…………………………………..….7.5 mg

Irgasan………………………………….……...2.0 mg

Novobiocine…………………..…………….1.25 mg



Formula
D-Mannitol 20
Enzymatic Digest of Gelatin 17
Enzymatic Digest of Casein and Animal Tissues 3
Yeast Extract 2
Sodium Pyruvate 2
Sodium Chloride 1
Sodium Desoxycholate 0.5
Neutral Red 0.03
Magnesium Sulfate Anhydrous 0.01
Crystal Violet 0.001
Bacteriological Agar 12.5